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Abstract

Background: Oocyte quality is an important factor in determining the success rates
of assisted conception cycles. We wanted to know the correlation of various oocyte
morphological and physical parameters with quality of the embryos.
Methods: This is a single institutional prospective observational study conducted after
institutional ethical committee approval over 18 months in 90 consenting women
undergoing in-vitro fertilization. Ovarian stimulation protocols, gonadotropin doses
and ovulation trigger were decided according to the patient’s clinical profile. After
retrieval, oocytes were incubated for 2 hours and denudation was done followed by
morphological assessment of each oocyte under inverted microscope and ICSI was
done with morphologically normal sperm. All injected oocytes were cultured singly
and the grade of embryo according to the Istanbul consensus for cleavage stage embryos
was noted for each oocyte.
Statistical Analysis/Results: 720 mature oocytes upon ICSI with morphological
sperm yielded 408 (56.6%) Grade A, 125 (17.4%) Grade B embryos which were
considered transferrable and 187 (26%) Grade C embryos and Unfertilized oocytes.
Each oocyte morphological parameter was studied against the grade of embryo it
formed.
Conclusion: We developed a mature oocyte morphological score with each variant of
the oocyte parameter for both significant and non-significant oocyte parameters.
Key words: Oocyte quality, Oocyte scoring, IVF outcomes

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